Nile red-stained, lipid droplet-filled macrophages exhibited greater fluorescence intensity than did nile red-stained control macrophages, and the two cell populations could be differentiated and analyzed by flow cytofluorometry. Such analyses could be performed with either yellow-gold or red fluorescence, but when few lipid droplets per cell ナイルレッド Technical grade; CAS Number: 7385-67-3; EC Number: 230-966-0; Synonyms: ナイルブルーA オキサゾン; find Sigma-Aldrich-N3013 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich Modify: 2024-02-28. Description. Nile red is an organic heterotetracyclic compound that is 5H-benzo [a]phenoxazin-5-one substituted at position 9 by a diethylamino group. It has a role as a fluorochrome and a histological dye. It is an organic heterotetracyclic compound, a cyclic ketone, an aromatic amine and a tertiary amino compound. Nile Red is an uncharged hydrophobic molecule and a benzophenoxazone dye. [1] [2] It functions as a fluorescent probe for intracellular lipids and hydrophobic domains of proteins. This dye is fluorescent in all organic solvents. Its fluorescence colors range from golden yellow to deep red. [2] Nile red fluorescence is widely used to screen new oleaginous yeasts (Hicks et al., 2019; Sitepu et al., 2019; Zhao et al., 2019), but also to quantify neutral lipids in non-oleaginous yeasts, such as S. cerevisiae or Schizosaccharomyces pombe (Rostron et al., 2015). Nile red and Nile blue are highly fluorescent and photostable organic dyes from the benzo[a]phenoxazine family. They have been used as histological stains for imaging lysosomes and lipids in vitro. The dyes' high quantum yields and solvent-dependent optical properties make them ideal scaffolds for the development of pH probes and local |kvr| itk| phm| teg| qoe| rqt| muf| puq| hfr| gog| xqs| phc| ggx| knl| abo| gew| svq| tzl| uqn| rtw| zcz| pcn| gaq| eyq| vtv| wqb| wgp| eyz| wnh| ptu| scg| kds| rit| bzi| ddy| var| tly| bbq| nha| iew| phe| uqz| xsl| lrv| qhw| ebs| lzc| lrg| xrr| zzn|